Insertion junction: CLIP2.038699_2

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:+
Strain:CLIP2.038699
Chromosome:chromosome 13
Location:2260011
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre13.g578501 (1 of 1) IPR009060//IPR015940//IPR026741//IPR026937//IPR027417 - UBA-like // Ubiquitin-associated domain // Protein strawberry notch // Strawberry notch, helicase C domain // P-loop containing nucleoside triphosphate hydrolase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GGCCGCTGAAACGCGTTTTGCCGTGTTACATAGACACATACACTGATACA
Internal bar code:AGGTTGAAAACGTGGAGAGCAC

Confirmation - LEAP-Seq

LEAP-Seq distance:543
LEAP-Seq percent confirming:92.0
LEAP-Seq n confirming:23
LEAP-Seq n nonconfirming:2
LEAP-Seq n unique pos:25

Suggested primers for confirmation by PCR

Suggested primer 1:GGTGAACACCGAAACACACG
Suggested primer 2:TTTTCCTGCGTTTTGCTCCG