Insertion junction: CLIP2.039190_2

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.039190
Chromosome:chromosome 13
Location:1250145
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre13.g570500 (1 of 2) PF13374//PF13424 - Tetratricopeptide repeat (TPR_10) // Tetratricopeptide repeat (TPR_12) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTGGGATTGGTGAACTGGTCAAGACACGCTCCCATACCATACCCACGAAA
Internal bar code:TGAAGAATAAGTTCGACGGGGA

Confirmation - LEAP-Seq

LEAP-Seq distance:964
LEAP-Seq percent confirming:12.5
LEAP-Seq n confirming:5
LEAP-Seq n nonconfirming:35
LEAP-Seq n unique pos:40

Suggested primers for confirmation by PCR

Suggested primer 1:GTTCGTGGTTCTGGTGTTGC
Suggested primer 2:ACCAGCCCGTACTAGGTCAT