| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.039380 |
| Chromosome: | chromosome 5 |
| Location: | 2350751 |
| Confidence (%): | 40 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g238332 | PSAD,PSAD1 | Photosystem I reaction center subunit II, 20 kDa; (1 of 1) K02692 - photosystem I subunit II (psaD) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGCAGCAGCTGGACCGCCTGTACCATGGAGAAGAGCTTTACTTGCCGGG |
| Internal bar code: | CTACACGGAGGACTATCTACGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 324 |
| LEAP-Seq percent confirming: | 66.6667 |
| LEAP-Seq n confirming: | 2 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGCAGTATGGCATTGGCAGA |
| Suggested primer 2: | AGGGGGATCTGGGAATTGGA |