Insertion junction: CLIP2.039615_2

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:-
Strain:CLIP2.039615
Chromosome:chromosome 17
Location:2326374
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre17.g713650 MSH5 DNA mismatch repair protein, MutS homolog; (1 of 1) K08741 - DNA mismatch repair protein MSH5 (MSH5) CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GGTTGAAGAGGCTGAAGCCCTCCTTGGGCCGGCCCAGCCCCATGGCCGAG
Internal bar code:ACAGGTATAAATTTGTTAGCTC

Confirmation - LEAP-Seq

LEAP-Seq distance:54
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:2
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:2

Suggested primers for confirmation by PCR

Suggested primer 1:AGGAAGGGGAGGAGGAAGAC
Suggested primer 2:ACACACACACACACACACCT