Insertion junction: CLIP2.039668_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.039668
Chromosome:chromosome 15
Location:465738
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre15.g635800 SMC1 Structural Maintenance of Chromosomes protein; (1 of 1) K06636 - structural maintenance of chromosome 1 (SMC1) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTGCGTGGGCCAGTTGTGAATGCGGGCACGCACACGTGTCACTACACCGA
Internal bar code:GGAGGATGCTGACGGCCGTTCG

Confirmation - LEAP-Seq

LEAP-Seq distance:183
LEAP-Seq percent confirming:62.5
LEAP-Seq n confirming:10
LEAP-Seq n nonconfirming:6
LEAP-Seq n unique pos:16

Suggested primers for confirmation by PCR

Suggested primer 1:TTGGTTCAGTTTGGGCTGGT
Suggested primer 2:GGTCCTTGTAGATGGCGGAG