Insertion junction: CLIP2.039750_3

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:+
Strain:CLIP2.039750
Chromosome:chromosome 12
Location:128199
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre12.g484200 GGPS,PPS2,GGPS1 Geranylgeranyl pyrophosphate synthase, putative chloroplast precursor; (1 of 1) 2.5.1.29 - Geranylgeranyl diphosphate synthase / Geranylgeranyl-PP synthetase 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CGAGGCCTGAGCTGTCGGCCGGTTCACGGGGCGGTCGCGGGGCGGTCGCG
Internal bar code:GTGAAATTGCACCTGGGGTAAC

Confirmation - LEAP-Seq

LEAP-Seq distance:688
LEAP-Seq percent confirming:96.1538
LEAP-Seq n confirming:25
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:26

Suggested primers for confirmation by PCR

Suggested primer 1:GCCTTCTCCTTCACCCACTC
Suggested primer 2:TTGTTTGTTGCCATGCTCCG