| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.039758 |
| Chromosome: | chromosome 12 |
| Location: | 5171569 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g526883 | (1 of 18) PF12906 - RING-variant domain (RINGv) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGTTTCCCACACCGAGCCCGCGGTGTGAGCCCCGGAGACGCCAGCCTGTC |
| Internal bar code: | ATTGAACTTGTATGATTTTTTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 417 |
| LEAP-Seq percent confirming: | 80.0 |
| LEAP-Seq n confirming: | 28 |
| LEAP-Seq n nonconfirming: | 7 |
| LEAP-Seq n unique pos: | 35 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGTGTTTGACCTAGTGGGGC |
| Suggested primer 2: | GCACGTTGATGTGATGGGTG |