| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | + |
| Strain: | CLIP2.039787 |
| Chromosome: | chromosome 6 |
| Location: | 944145 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g256350 | (1 of 3) 1.1.2.6 - Polyvinyl alcohol dehydrogenase (cytochrome) / PVADH | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTTGTAGACTGTGGGCGAGGCGGTGATGATGGCGAAGGGGTGGGAGTCGG |
| Internal bar code: | AAATAAATTAACATCGTTTCAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1096 |
| LEAP-Seq percent confirming: | 86.9565 |
| LEAP-Seq n confirming: | 20 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 23 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCAAGTTTGCGAAACCCTGG |
| Suggested primer 2: | CGACTGATGCATGCATGTGG |