| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.040469 |
| Chromosome: | chromosome 7 |
| Location: | 3498386 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g336050 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACGTGGAATGTGGTGGGGTTTCCATGCATGAGTAATTATGAGTTCAGAAC |
| Internal bar code: | TTGTGCGGTTTAAACGTCCTGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2278 |
| LEAP-Seq percent confirming: | 37.7358 |
| LEAP-Seq n confirming: | 20 |
| LEAP-Seq n nonconfirming: | 33 |
| LEAP-Seq n unique pos: | 53 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTGGGTCACACCGTCTCATT |
| Suggested primer 2: | ACGTCAACCTATAGGCCCCT |