| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.040684 |
| Chromosome: | chromosome 12 |
| Location: | 9354006 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g541800 | (1 of 2) 3.4.21.26 - Prolyl oligopeptidase / Prolyl endopeptidase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATGACGGAGTAAGAAAGCGGCTGCGACTGTTCGAGAAGCAGCGAGCCAGG |
| Internal bar code: | CAGTGATCTAAATAGTATCACG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 570 |
| LEAP-Seq percent confirming: | 93.75 |
| LEAP-Seq n confirming: | 15 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 16 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTTGTGTGCCTCCTCAGGTC |
| Suggested primer 2: | GCCGCTGTTGTGGTAGTAGT |