| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.040796 |
| Chromosome: | chromosome 10 |
| Location: | 4782370 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g452900 | SMY2 | (1 of 2) 3.1.4.12 - Sphingomyelin phosphodiesterase / Neutral sphingomyelinase; Sphingomyelin phosphodiesterase/sphingomylinase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AAGGGGTGAATCGGCGTGGCAGGAAAGGGAACATGGCCGCATGGTGCAGT |
| Internal bar code: | CGGGCCTAGAAGCCAGGGAAGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1473 |
| LEAP-Seq percent confirming: | 89.2857 |
| LEAP-Seq n confirming: | 50 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 56 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTAAATCGCACGGCCTTTCG |
| Suggested primer 2: | GGCATATAGAACCGCCACGA |