| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.040881 |
| Chromosome: | chromosome 14 |
| Location: | 2013892 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g621000 | SPL27 | Pre-mRNA splicing factor; (1 of 1) K12869 - crooked neck (CRN, CRNKL1, CLF1, SYF3) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCCTGTTTACGCTGCCTTAACCCCGCAACCGCCACCCCGAACCCCTCCGT |
| Internal bar code: | AGGGGTGGGGCTTTGACAAAGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2176 |
| LEAP-Seq percent confirming: | 85.7143 |
| LEAP-Seq n confirming: | 24 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 28 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTCCTCCTGCTTCTCCTCCT |
| Suggested primer 2: | GTGTGTCGGAGGTGGAGAAG |