Insertion junction: CLIP2.041057_2

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:+
Strain:CLIP2.041057
Chromosome:chromosome 11
Location:1666068
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre11.g468356 TPR7,TPR (1 of 1) K09291 - nucleoprotein TPR (TPR, MLP1, MLP2); Tetratricopeptide-repeat protein 7 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ACGGACATCGGTAACAACGCGTCTACGCTGCAACTGTACCTGTCTACGAC
Internal bar code:TAATTGGTCATGTGAAGAGTAG

Confirmation - LEAP-Seq

LEAP-Seq distance:92
LEAP-Seq percent confirming:72.7273
LEAP-Seq n confirming:8
LEAP-Seq n nonconfirming:3
LEAP-Seq n unique pos:11

Suggested primers for confirmation by PCR

Suggested primer 1:TTCTTGCGGATAAGGGCGTT
Suggested primer 2:AGGTCTACTTCTTGCACGGC