Insertion cassette: | CIB2 |
Side of cassette: | 5' |
Strand: | - |
Strain: | CLIP2.041082 |
Chromosome: | chromosome 12 |
Location: | 4881974 |
Confidence (%): | 80 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre12.g523850 | MPPB,QCR1 | (1 of 1) K17732 - mitochondrial-processing peptidase subunit beta [EC:3.4.24.64] (PMPCB, MAS1); Ubiquinol:cytochrome c oxidoreductase 50 kDa core 1 subunit | 3'UTR |
Cre12.g801416 | (1 of 13) PF12847 - Methyltransferase domain (Methyltransf_18) | intron |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CAGGCCCGCCGCCGTCCCCTTGGCGCCCACCTGAGATGTCCTCGGCCGCA |
Internal bar code: | TGTATGTAGCTGTATGGTTGGA |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 3176 |
LEAP-Seq percent confirming: | 100.0 |
LEAP-Seq n confirming: | 43 |
LEAP-Seq n nonconfirming: | 0 |
LEAP-Seq n unique pos: | 43 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | TGTTGATGGGTGTGGTACGG |
Suggested primer 2: | AAGGGCGAGGTAGGAGATGT |