| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.041295 |
| Chromosome: | chromosome 2 |
| Location: | 1803871 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g086850 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATTACAATGCTTTCTTGTGTGTTTGTGCCCCAACACAGAACTTTGTGGAC |
| Internal bar code: | GTTCGGGGAATCTACGCTTCGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2059 |
| LEAP-Seq percent confirming: | 84.7458 |
| LEAP-Seq n confirming: | 50 |
| LEAP-Seq n nonconfirming: | 9 |
| LEAP-Seq n unique pos: | 59 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTCAAGTGGACGGTCGGAAG |
| Suggested primer 2: | GGTTGGGTCACAGGAGATGG |