| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.041729 |
| Chromosome: | chromosome 13 |
| Location: | 3648895 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g588150 | VTC2 | GDP-L-galactose phosphorylase; (1 of 1) 2.7.7.69 - GDP-L-galactose phosphorylase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATTCGCCGCCGCCCACCGGCTTGGCCCTGCCGGAGTACGGGCTTGGCGTG |
| Internal bar code: | CATCCGCGAGGGGCTGATTTCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1303 |
| LEAP-Seq percent confirming: | 70.0 |
| LEAP-Seq n confirming: | 7 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGCATGGTAAGAAGCGAGGG |
| Suggested primer 2: | GGTGGCCAGTAACCTTCCTC |