Insertion junction: CLIP2.041905_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.041905
Chromosome:chromosome 1
Location:5568753
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre01.g039650 PDF1C (1 of 3) 3.5.1.88 - Peptide deformylase / Polypeptide deformylase; Peptide deformylase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ATCGGGTTCCGCCTCCGCTGCACCTGCCACCGCAGCCTCGGGAGCTGCTG
Internal bar code:CTTGGCAAACGTCAATATGTAT

Confirmation - LEAP-Seq

LEAP-Seq distance:1602
LEAP-Seq percent confirming:75.6757
LEAP-Seq n confirming:28
LEAP-Seq n nonconfirming:9
LEAP-Seq n unique pos:37

Suggested primers for confirmation by PCR

Suggested primer 1:ACCGCTTCAGCTGTTACACA
Suggested primer 2:TCAAAGTTCAGGGTCGGTCG