| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.042852 |
| Chromosome: | chromosome 13 |
| Location: | 2714357 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g581801 | (1 of 1) PTHR13198//PTHR13198:SF4 - RING FINGER PROTEIN 25 // E3 UBIQUITIN-PROTEIN LIGASE RNF25 | intron | |
| Cre13.g581850 | (1 of 1) PTHR10566//PTHR10566:SF53 - CHAPERONE-ACTIVITY OF BC1 COMPLEX CABC1 -RELATED // SUBFAMILY NOT NAMED | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGCCCTGCAGCTCCCTGTGATCGACACCAGGCATGGTAACTGGTGATGAG |
| Internal bar code: | TTAGCATTCCCCCTTTAAACCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2532 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 63 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 63 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGCGGTGCAAACAGTTTACG |
| Suggested primer 2: | GGTCATGGTCATGGTGGCA |