Insertion junction: CLIP2.042894_2

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.042894
Chromosome:chromosome 12
Location:9320277
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre12.g542100 (1 of 2) 3.4.21.26 - Prolyl oligopeptidase / Prolyl endopeptidase CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCACGCGGTCGTCGTGGTCGCCTGGAGGAGAGGGCAGGCAGGAGCGTGGG
Internal bar code:GTACACCCTCTGAGGGGAATGA

Confirmation - LEAP-Seq

LEAP-Seq distance:1621
LEAP-Seq percent confirming:86.8421
LEAP-Seq n confirming:33
LEAP-Seq n nonconfirming:5
LEAP-Seq n unique pos:38

Suggested primers for confirmation by PCR

Suggested primer 1:CCCACAACAAGCAGAACGTG
Suggested primer 2:TTTGGCGTTCATGCACTTGG