Insertion junction: CLIP2.042925_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.042925
Chromosome:chromosome 11
Location:341604
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre11.g467575 CLPB4 (1 of 1) PF07728//PF10431//PF13191 - AAA domain (dynein-related subfamily) (AAA_5) // C-terminal, D2-small domain, of ClpB protein (ClpB_D2-small) // AAA ATPase domain (AAA_16); ClpB chaperone, Hsp100 family intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTTTGACTTCACCGGGCGCGGCGCCTCGCTGCCTCATCACCCGCCCCTAC
Internal bar code:ACAAGGATGTCTAGGTGTTCGT

Confirmation - LEAP-Seq

LEAP-Seq distance:223
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:2
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:2

Suggested primers for confirmation by PCR

Suggested primer 1:ACGATACCTCTTGCAGCCAC
Suggested primer 2:CTTCCCCTGATGCCACATGT