Insertion junction: CLIP2.043709_2

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:+
Strain:CLIP2.043709
Chromosome:chromosome 16
Location:4057581
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre16.g690400 DNJ5 (1 of 62) PF00226 - DnaJ domain (DnaJ); DnaJ-like protein CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTCAGGATCGGGGCGAGGCGGAGCGGCAGTGGGAGGCGCACCACGCTGGC
Internal bar code:TGCTATTTTCTATCGTAGGTGT

Confirmation - LEAP-Seq

LEAP-Seq distance:1741
LEAP-Seq percent confirming:81.8182
LEAP-Seq n confirming:9
LEAP-Seq n nonconfirming:2
LEAP-Seq n unique pos:11

Suggested primers for confirmation by PCR

Suggested primer 1:GTGTGAAAAAGCGCGAGTGT
Suggested primer 2:AGCTGTCTTATCATCGCGGG