| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.043986 |
| Chromosome: | chromosome 12 |
| Location: | 1268574 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g489700 | OTC1 | Ornithine carbamoyltransferase; (1 of 1) 2.1.3.3 - Ornithine carbamoyltransferase / OTCase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCGTAACTGATCTTCTCGTTCACCGTACCGCGACACCAGCCCGCATTCGC |
| Internal bar code: | AGCGAGGATGTGTATGGTTGAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1882 |
| LEAP-Seq percent confirming: | 94.6667 |
| LEAP-Seq n confirming: | 71 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 75 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGCTACCAGGGGTATCCGAA |
| Suggested primer 2: | TACAGCGGTAAAAGCCCCAG |