Insertion junction: CLIP2.044065_4

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:-
Strain:CLIP2.044065
Chromosome:chromosome 13
Location:2514978
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre13.g580000 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TCACAGCTGATGGCTTGTCAGGTCTAGGCAGGAGATAACTCACGTGGAGA
Internal bar code:ATGCTTCCGACTCAGTACGCAA

Confirmation - LEAP-Seq

LEAP-Seq distance:2479
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:40
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:40

Suggested primers for confirmation by PCR

Suggested primer 1:CGTTTACAGCATCCTTCGCG
Suggested primer 2:TTTCCGTCAGTTGGTCCGAG