Insertion junction: CLIP2.044081_2

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.044081
Chromosome:chromosome 6
Location:944888
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre06.g256350 (1 of 3) 1.1.2.6 - Polyvinyl alcohol dehydrogenase (cytochrome) / PVADH intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GATACGGACGCGTGAGGAGGGTGGAAGCTAGGACGGGGATGTGTACGGGG
Internal bar code:GGCGGTCGATAAAGGCAGTGTA

Confirmation - LEAP-Seq

LEAP-Seq distance:1887
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:65
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:65

Suggested primers for confirmation by PCR

Suggested primer 1:GATCTTACCGGTGTTGCCCA
Suggested primer 2:AGGTACGGGCTGTGTAGAGT