Insertion junction: CLIP2.044276_2

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.044276
Chromosome:chromosome 13
Location:1253808
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre13.g570500 (1 of 2) PF13374//PF13424 - Tetratricopeptide repeat (TPR_10) // Tetratricopeptide repeat (TPR_12) CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ATTTCGGAGAGCTTGTCAGTGTCATCCTCGACATCCCAGAGACGGATGGC
Internal bar code:CCTCAATTGCGATATCTAACAA

Confirmation - LEAP-Seq

LEAP-Seq distance:1358
LEAP-Seq percent confirming:62.5
LEAP-Seq n confirming:5
LEAP-Seq n nonconfirming:3
LEAP-Seq n unique pos:8

Suggested primers for confirmation by PCR

Suggested primer 1:TCTCCTGCACGTTTCACCTC
Suggested primer 2:GGACCGACGAGTTCACACTT