| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.044562 |
| Chromosome: | chromosome 1 |
| Location: | 5253932 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g036850 | PDY1 | (1 of 2) 1.5.5.2 - Proline dehydrogenase; Proline dehydrogenase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTGTACCCAAACGTACGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGT |
| Internal bar code: | TCTTGTCACATAAGTGATCATT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1751 |
| LEAP-Seq percent confirming: | 85.3659 |
| LEAP-Seq n confirming: | 35 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 41 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCGCGTCAATCATCAGCTTG |
| Suggested primer 2: | CCCAAGAGCTCTGCATCACA |