| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.045319 |
| Chromosome: | chromosome 10 |
| Location: | 1600284 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g429100 | Putative prolyl-tRNA synthetase; (1 of 1) PTHR11451:SF21 - BIFUNCTIONAL GLUTAMATE/PROLINE--TRNA LIGASE | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTTGCGCGCGCGACCCACCCTCTCCCTCCGCCTTAGCCTCCTTCTTCGGC |
| Internal bar code: | TTTCAGCCCATAAGTACCGCTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3219 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 63 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 63 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGGTGCGCTACTGGAAATGT |
| Suggested primer 2: | GTCTCCTCCCACCCACAATG |