Insertion junction: CLIP2.045404_2

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.045404
Chromosome:chromosome 7
Location:1208131
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre07.g321400 MAW6,FAP113 (1 of 1) PF13948 - Domain of unknown function (DUF4215) (DUF4215); Membrane-associated hydroxyproline-rich glycoprotein 6 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TTAAGATGTGCACAGGGGAGTAGCAGGGGGTTGGCGGCGCGGGGTTCACA
Internal bar code:CATTAGATGAAGATCATTTATG

Confirmation - LEAP-Seq

LEAP-Seq distance:2225
LEAP-Seq percent confirming:86.6667
LEAP-Seq n confirming:13
LEAP-Seq n nonconfirming:2
LEAP-Seq n unique pos:15

Suggested primers for confirmation by PCR

Suggested primer 1:CTTTTTGCTGTGCGTCTGCT
Suggested primer 2:CGGTTGTTCTTGCACACCTG