| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.045600 |
| Chromosome: | chromosome 12 |
| Location: | 7721733 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g554850 | TRXH2,TRX7,TRXh2,TRXh2a | (1 of 9) K03671 - thioredoxin 1 (trxA); Thioredoxin h2, cytosolic | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCGCCCCGCTCTAGTGAGTAACGCCGCAGCGAGGCTCGGCGCAGGATGCG |
| Internal bar code: | CATACTTTTTTCAGGAGAAGTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 312 |
| LEAP-Seq percent confirming: | 59.0909 |
| LEAP-Seq n confirming: | 13 |
| LEAP-Seq n nonconfirming: | 9 |
| LEAP-Seq n unique pos: | 22 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AATACCTGCTCGCCGTTGAA |
| Suggested primer 2: | GGAGCAACTCGCAACACTTG |