Insertion junction: CLIP2.045772_2

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.045772
Chromosome:chromosome 4
Location:681222
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre04.g217951 LCI33 Low-CO2-inducible protein; (1 of 9) IPR011011//IPR013083 - Zinc finger, FYVE/PHD-type // Zinc finger, RING/FYVE/PHD-type CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TCGCACCCGCCAGGCTTATGCCAAGGGCCTGACTGTCCCCCAGGGACACC
Internal bar code:AGGGTGTAGTAATGATTGGATT

Confirmation - LEAP-Seq

LEAP-Seq distance:3375
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:73
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:73

Suggested primers for confirmation by PCR

Suggested primer 1:GCTTGGACACACTGCTGTTG
Suggested primer 2:GGCCACATCTCCAAATCCCA