| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.046284 |
| Chromosome: | chromosome 6 |
| Location: | 4058042 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g278221 | TSSP1,TPS1,TSPSP1 | (1 of 1) 2.4.1.15//3.1.3.12 - Alpha,alpha-trehalose-phosphate synthase (UDP-forming) / UDP-glucose--glucose-phosphate glucosyltransferase // Trehalose-phosphatase / Trehalose 6-phosphate phosphatase; Trehalose-6-phosphate synthase/phosphatase, class I | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGCCCGCTGTCAGGACTTGGCCGCCCACTCGCAACCTCGCGACTGAGCCA |
| Internal bar code: | TTTTAGCCCGTTTATGCCCTGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1423 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 27 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 27 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAAACCGAAACCAACCCTGC |
| Suggested primer 2: | AAGCAGTCTAGCCAGCACAG |