| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.046410 |
| Chromosome: | plastome |
| Location: | 58700 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| CreCp.g802286 | ycf12 | proposed transmembrane component of photosystem II; (1 of 1) PF05969 - Photosystem II complex subunit Ycf12 (PSII_Ycf12) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACTAGTGTAAGAGCTAATTCAATATTCATATGAAAAATTTTTTTTTATAT |
| Internal bar code: | GACGGATTATTTGGATATGACT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 4509 |
| LEAP-Seq percent confirming: | 81.8182 |
| LEAP-Seq n confirming: | 18 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 22 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACACCAGAGCGTCCTTTCTG |
| Suggested primer 2: | GTTCGTAGCCAGACCACACA |