| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.046486 |
| Chromosome: | chromosome 6 |
| Location: | 5484479 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g286250 | MPC1,MSCP1 | (1 of 4) K15111 - solute carrier family 25 (mitochondrial S-adenosylmethionine transporter), member 26 (SLC25A26); Mitochondrial substrate carrier protein | 3'UTR |
| Cre06.g286300 | UMM5,COQ5B | (1 of 2) PTHR10108//PTHR10108:SF830 - METHYLTRANSFERASE // SUBFAMILY NOT NAMED; Ubiquinone/menaquinone biosynthesis methyltransferase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGAGGGGGTGCGGGGACATTGCTATCTGCAACAGTGCCACGTTGCCGGGT |
| Internal bar code: | ATCCGGCAGCTCCATCATGCTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2689 |
| LEAP-Seq percent confirming: | 91.1765 |
| LEAP-Seq n confirming: | 62 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 68 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGACACCAAGTACCTGTGCT |
| Suggested primer 2: | TGGTGGAAGGCAGGTGTTTT |