| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | + |
| Strain: | CLIP2.046506 |
| Chromosome: | chromosome 12 |
| Location: | 9322145 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g542050 | CDS | ||
| Cre12.g542100 | (1 of 2) 3.4.21.26 - Prolyl oligopeptidase / Prolyl endopeptidase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGGCGTGTCGGACTGCTGCTGCTTGCTGCTGCCGCGCATGTAGAGCGGCA |
| Internal bar code: | AAATGGTACAGGTGAAACTTCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 886 |
| LEAP-Seq percent confirming: | 36.8421 |
| LEAP-Seq n confirming: | 7 |
| LEAP-Seq n nonconfirming: | 12 |
| LEAP-Seq n unique pos: | 19 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCAGTCCCTACGTTCACACA |
| Suggested primer 2: | GCAACTGAAACCTGTGCTGG |