Insertion junction: CLIP2.046524_4

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.046524
Chromosome:chromosome 12
Location:6984243
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre12.g560900 CPLD35,AOF7 Flavin-containing amine oxidase. Conserved in plant lineage and Diatoms; (1 of 2) 1.3.5.5 - 15-cis-phytoene desaturase / Plant-type phytoene desaturase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTACCCCACCTGTCTACCCCACCCGGTGTTCAACCCTTCCTGCACCCTCA
Internal bar code:ATGAGTTTACTTGGTTTCTCT

Confirmation - LEAP-Seq

LEAP-Seq distance:3265
LEAP-Seq percent confirming:82.1429
LEAP-Seq n confirming:92
LEAP-Seq n nonconfirming:20
LEAP-Seq n unique pos:112

Suggested primers for confirmation by PCR

Suggested primer 1:ACTACCATAAGGAGGCCGGT
Suggested primer 2:ACCTGCAACCTGTACTTCCG