Insertion junction: CLIP2.046658_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.046658
Chromosome:chromosome 1
Location:821243
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre01.g004550 FAP190 (1 of 7) 2.7.10.2//2.7.12.1 - Non-specific protein-tyrosine kinase / Cytoplasmic protein tyrosine kinase // Dual-specificity kinase; Flagellar Associated Protein 190 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GGTTAGGAACGGTCGCACAGGCGTGCTACACCACACCCAAGGCCCCGCCC
Internal bar code:CTTGGGTAGCCAATGCGAGAGT

Confirmation - LEAP-Seq

LEAP-Seq distance:3629
LEAP-Seq percent confirming:53.125
LEAP-Seq n confirming:34
LEAP-Seq n nonconfirming:30
LEAP-Seq n unique pos:64

Suggested primers for confirmation by PCR

Suggested primer 1:CCTCCCATCCAGTCCAACAC
Suggested primer 2:CCTTTGTTTGGACTCGCTGC