Insertion junction: CLIP2.046956_3

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.046956
Chromosome:chromosome 10
Location:2044338
Confidence (%):63
Locus systematic id Locus common name Defline Feature
Cre10.g432550 KCN2 Six-transmembrane domain potassium channel alpha subunit; (1 of 1) PTHR11537:SF133 - PROTEIN EXP-2, ISOFORM C intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTGTCCCGCTGCACCCCACACGCCGCGTATGTTCGCTCATGCTCACACCC
Internal bar code:ATCATCTAGAGGTGTGTCTTTA

Confirmation - LEAP-Seq

LEAP-Seq distance:2912
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:1
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:1

Suggested primers for confirmation by PCR

Suggested primer 1:GACGATGCAGAGCGAGATGA
Suggested primer 2:GATCGAGACGGTGACCATCC