Insertion junction: CLIP2.047075_2

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.047075
Chromosome:chromosome 6
Location:6189720
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre06.g292000 (1 of 1) PF00790//PF03127 - VHS domain (VHS) // GAT domain (GAT) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ACGCAAGCTCCGGACATGGAAACGATCGCCGAGAAACAACACCCATCTCT
Internal bar code:AGCCGGTCGGTTACCGGTAAAC

Confirmation - LEAP-Seq

LEAP-Seq distance:2135
LEAP-Seq percent confirming:96.8254
LEAP-Seq n confirming:61
LEAP-Seq n nonconfirming:2
LEAP-Seq n unique pos:63

Suggested primers for confirmation by PCR

Suggested primer 1:GTTGACGGTTTCAAACGGGG
Suggested primer 2:TACAACGCGCTTGACACTCT