| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.047148 |
| Chromosome: | chromosome 3 |
| Location: | 6754258 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g196450 | (1 of 1) K15178 - RNA polymerase-associated protein RTF1 (RTF1) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CAGGAACTGTGCACCAAGTTATTGCCTGCGTGTTGACAGGCCGCTGAGGA |
| Internal bar code: | TTGGATAGTCAGACGGAAGACA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3230 |
| LEAP-Seq percent confirming: | 97.2973 |
| LEAP-Seq n confirming: | 36 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 37 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAAGCAGGGGAGGAAAGGAC |
| Suggested primer 2: | ACTGGGACTGGTGCGTTATG |