| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.047251 |
| Chromosome: | chromosome 6 |
| Location: | 7242716 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g298850 | LTH6 | LAT2 homolog | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGGAGAGAGTGTAGGAGGGCCCACTGCGGCAGCGCCGGCCGCGGCAGCTC |
| Internal bar code: | TCCGAGAACAAAGAGATCCGAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3139 |
| LEAP-Seq percent confirming: | 75.0 |
| LEAP-Seq n confirming: | 6 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATGTGGTGGACATCAGCTGG |
| Suggested primer 2: | AAGGACATGGTGCTAAGGCC |