| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.048106 |
| Chromosome: | chromosome 3 |
| Location: | 4889707 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g178600 | TOP1 | DNA topoisomerase I; (1 of 1) K03163 - DNA topoisomerase I [EC:5.99.1.2] (TOP1) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TACATGGACCCGCGCATCACCGTGGCCTGGTGCAAGCGCAACGAGGTGCC |
| Internal bar code: | GCACCTAATACAAGAGCGGTGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1328 |
| LEAP-Seq percent confirming: | 70.3704 |
| LEAP-Seq n confirming: | 19 |
| LEAP-Seq n nonconfirming: | 8 |
| LEAP-Seq n unique pos: | 27 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCCTATCCGTACAACCGCTC |
| Suggested primer 2: | GCACACCAACCAGATGGAGA |