Insertion junction: CLIP2.048162_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.048162
Chromosome:chromosome 8
Location:461716
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre08.g358950 (1 of 1) PF09731 - Mitochondrial inner membrane protein (Mitofilin) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TCCTTCAGCTCCAGCCCTTCTACTCCCCTCCCCCTCCTTCCGATCGCCTA
Internal bar code:TGTGCGGAACGTTACATGTCCG

Confirmation - LEAP-Seq

LEAP-Seq distance:261
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:5
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:5

Suggested primers for confirmation by PCR

Suggested primer 1:ACATGTGACACGTGCTCCAA
Suggested primer 2:AAGTCCACTGTGCATACCCG