| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.048174 |
| Chromosome: | chromosome 10 |
| Location: | 3813545 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g445700 | (1 of 55) PTHR23257//PTHR23257:SF394 - SERINE-THREONINE PROTEIN KINASE // SUBFAMILY NOT NAMED | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCTGAACGTCAGCATGGTTACCGCTGCCGTCGCCGCCTTGCGTCACGCT |
| Internal bar code: | CGCCGGTCGATGCCTTCATTTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3639 |
| LEAP-Seq percent confirming: | 58.75 |
| LEAP-Seq n confirming: | 47 |
| LEAP-Seq n nonconfirming: | 33 |
| LEAP-Seq n unique pos: | 80 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TACCCGATGATGATGCCTGC |
| Suggested primer 2: | GCTCCCATGGGATTATGCGA |