| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | + |
| Strain: | CLIP2.048389 |
| Chromosome: | chromosome 12 |
| Location: | 3617293 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g513200 | PGH1,ENO1,ENO | (1 of 1) 4.2.1.11 - Phosphopyruvate hydratase / Enolase; Enolase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGGGGAAGATACCCTTAACTGCTTCTCGTTGTTTCCAGGTACCTGGGCA |
| Internal bar code: | GCGTTCAAGTATTTCGATGCCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 4373 |
| LEAP-Seq percent confirming: | 97.619 |
| LEAP-Seq n confirming: | 123 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 126 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTTGCCCTTGTTGTCAGTGC |
| Suggested primer 2: | GCATTGGATCACCGAATGCC |