Gene: Cre12.g513200
Overview
| Common name: | PGH1,ENO1,ENO |
| Defline: | (1 of 1) 4.2.1.11 - Phosphopyruvate hydratase / Enolase; Enolase |
| Description: | Phosphoenolpyruvate hydratase; 2-phosphoglycerate dehydratase; EC 4.2.1.11 [GI:18143, Dumont et al. (1993) Plant Sci. 89, 55-67]; product localization unsure: an N-terminal extension also found in Dunaliella and At1g74030 potentially targets it to an organelle, especially if cDNA is extended >6 nt upstream; found in the flagellar proteome [PMID: 15998802] and associated with central pair projection C1b (PMID: 16030251). |
| Synonyms: | ENO,PGH1,Cre12.g513200.t1.1,g12671.t1 |
| Chromosome: | chromosome 12 |
Protein Localization
A plasmid for expressing Cre12.g513200 tagged with the fluorescent protein Venus and affinity epitope 3xFLAG is available: pLM005-Cre12.g513200-Venus-3xFLAG
Imaged strain: CSI_LW09C5
Imaging conditions: Tris-Phosphate media, air, 150 uM photons/m2/s, shaking in 96-well plate; imaged after 16 hours.
Classification(s):
cytosol/cytosol (strong signal)
nucleus/nucleoplasm
nucleus/nucleolus
secretory pathway/contractile vacuole
If you reference these protein localization data in a manuscript, please cite: Wang et al. 2023.
 |  |  |  |
| Venus | Chlorophyll | Bright field | Merged |
Protein-Protein Interactions
We did not determine any protein-protein interactions for this gene.
Mutant Strains
| Insertion Junction | Mutant Strain | Insertion Junction Feature | Confidence (%) | Genome Version |
|---|---|---|---|---|
| CLIP2.042688_1 | CLIP2.042688 | intron | 80 | 6.1 |
| CLIP2.048389_1 | CLIP2.048389 | intron | 80 | 6.1 |
| CLIP2.060611_1 | CLIP2.060611 | 5'UTR | 80 | 6.1 |
| LMJ.RY0402.044202_1 | LMJ.RY0402.044202 | 3'UTR | 73 | 5.5 |
| LMJ.RY0402.163904_1 | LMJ.RY0402.163904 | 3'UTR | 58 | 5.5 |
| LMJ.RY0402.167378_1 | LMJ.RY0402.167378 | 3'UTR | 95 | 5.5 |
| LMJ.RY0402.167378_2 | LMJ.RY0402.167378 | 3'UTR | 95 | 5.5 |
| LMJ.RY0402.193753_1 | LMJ.RY0402.193753 | 3'UTR | 95 | 5.5 |
| LMJ.RY0402.193753_2 | LMJ.RY0402.193753 | 3'UTR | 95 | 5.5 |
| LMJ.RY0402.199056_1 | LMJ.RY0402.199056 | 5'UTR | 73 | 5.5 |
Gene Annotations
| PFAM: | PF00113, PF03952 |
| PANTHER: | PTHR11902 |
| KEGG_ec: | EC:4.2.1.11 |
| KEGG_orthology: | K01689 |
| KOG: | KOG2670 |
| Gene ontology terms:: | GO:0000015, GO:0000287, GO:0004634, GO:0006096 |
| Best arabidopsis TAIR name: | AT2G36530 |
| Best arabidopsis TAIR symbol: | ENO2,LOS2 |
| Best arabidopsis TAIR defline: | Enolase |
| Phytozome Locus Page |
Phenotypes
Our phenotype screens did not find statistically significant results for this gene.