| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.193753 |
| Chromosome: | chromosome 12 |
| Location: | 3525475 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g513200 | PGH1,ENO1,ENO | (1 of 1) 4.2.1.11 - Phosphopyruvate hydratase / Enolase; Enolase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATAAGGACTTCTGCGGCGTTCAGGGCGGAC |
| Internal bar code: | GTCCGCCGGCCCCCTCTATCCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 464 |
| LEAP-Seq percent confirming: | 88.75 |
| LEAP-Seq n confirming: | 1988 |
| LEAP-Seq n nonconfirming: | 252 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTTACTCATGGTGGCCGAGT |
| Suggested primer 2: | GGGAAGCTACAGCTCCTCCT |