Insertion cassette: | CIB1 |
Side of cassette: | 5' |
Strand: | - |
Strain: | LMJ.RY0402.163904 |
Chromosome: | chromosome_12 |
Location: | 3525361 |
Confidence (%): | 58 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre12.g513200 | PGH1 | Enolase | antisense | 3'UTR |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | ATAAGGACTTCTGCGGCGTTCAGGGCGGAC |
Internal bar code: | CAGGTAGTGGTATATAATTCTT |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 829 |
LEAP-Seq percent confirming: | 99.1736 |
LEAP-Seq n confirming: | 480 |
LEAP-Seq n nonconfirming: | 4 |
LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | ATCCTTCTCCAACGCTGCTA |
Suggested primer 2: | CTGAGGACTCTTTCATCGCC |