Insertion junction: LMJ.RY0402.044202_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.044202
Chromosome:chromosome_12
Location:3525513
Confidence (%):73
Locus systematic id Locus common name Defline Orientation Feature
Cre12.g513200 PGH1 Enolase sense 3'UTR

Insertion site details

Flanking sequence (orientation from cassette outwards):CTTACACAGTCCGCCCTGAACGCCGCAGAA
Internal bar code:GATCTGCATAGCAAGACTGCAG

Confirmation - LEAP-Seq

LEAP-Seq distance:591
LEAP-Seq percent confirming:99.7359
LEAP-Seq n confirming:6797
LEAP-Seq n nonconfirming:18
LEAP-Seq n unique pos:5

Suggested primers for confirmation by PCR

Suggested primer 1:GTTACTCATGGTGGCCGAGT
Suggested primer 2:GGGAAGCTACAGCTCCTCCT