Insertion cassette: | CIB1 |
Side of cassette: | 5' |
Strand: | + |
Strain: | LMJ.RY0402.044202 |
Chromosome: | chromosome_12 |
Location: | 3525513 |
Confidence (%): | 73 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre12.g513200 | PGH1 | Enolase | sense | 3'UTR |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | CTTACACAGTCCGCCCTGAACGCCGCAGAA |
Internal bar code: | GATCTGCATAGCAAGACTGCAG |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 591 |
LEAP-Seq percent confirming: | 99.7359 |
LEAP-Seq n confirming: | 6797 |
LEAP-Seq n nonconfirming: | 18 |
LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | GTTACTCATGGTGGCCGAGT |
Suggested primer 2: | GGGAAGCTACAGCTCCTCCT |