| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.048478 |
| Chromosome: | chromosome 5 |
| Location: | 879236 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g246377 | ADA2 | Adenosine/AMP deaminase; (1 of 1) 3.5.4.2 - Adenine deaminase / Adenine aminase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCCTGCCGCCGCCCTACCCCTCCGTGGAGGCCGCCAGGTGGGTGGGGAGG |
| Internal bar code: | GACAACAATAGGAATATACGAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 367 |
| LEAP-Seq percent confirming: | 75.0 |
| LEAP-Seq n confirming: | 3 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTGTGCTCCGCTCGTACTAG |
| Suggested primer 2: | GTTCTGCGTCGGCTTTCATC |