| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.048536 |
| Chromosome: | chromosome 10 |
| Location: | 1579478 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g429017 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCGGTGCGGCCGCAGCGTGCCCGTCGCTGCCGGGAGTTTCTGGGGCGTTG |
| Internal bar code: | TATGCTTGCTATAGCCAGACAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3495 |
| LEAP-Seq percent confirming: | 81.1321 |
| LEAP-Seq n confirming: | 43 |
| LEAP-Seq n nonconfirming: | 10 |
| LEAP-Seq n unique pos: | 53 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGCGAAGATGTCTGCAGAGG |
| Suggested primer 2: | GTGCTAGCGATCCTGAGGAC |